[I-YS-03_04] 優秀発表賞演題(第Ⅰ会場)

[IYS-04E] Advanced in vitro evaluation system for selecting anti-diarrheal immunobiotics

^○小松 稜弥^1,2, 飯田 ひかる^1,2, Leonardo Albarracín³, Md. Aminul Islam^1,2, 大坪 和香子^1,2, 麻生 久^1,2, 岩渕 紀介⁴, 清水（肖） 金忠⁴, 須田 義人⁵, 齋藤 忠夫¹, Julio Villena³, 北澤 春樹^1,2 (1.東北大院農, 2.食と農免疫国際教育研究セ, 3.アルゼンチン国立乳酸菌研, 4.森永乳業(株), 5.宮城大)

[Objective] Several immunoregulatory probiotics (immunobiotics) are likely effective against intestinal infections. This study was to establish an advanced evaluation system for selecting anti-diarrheal immunobiotics for porcine host. [Methods] Porcine intestinal epithelial (PIE) cells were stimulated with 14 strains of lactic acid bacteria (LAB) individually followed by challenge with ETEC or poly (I:C), and measuring gene expressions by qPCR. Immune-related phenotypes were ranked by correlation analyses. High-ranked phenotypes were then assessed in ETEC-challenged PIE after the stimulation with 7 selected LAB strains. Host gene expressions were also compared to the genomic segments of LAB species. [Results]Immunobiotic stimulation differently affected the expression of immune related genes (phenotypes) in PIE cells. Some phenotypes had high correlations. Results suggested that not only chemical components but also physical factors of LAB genomes are important for immunobiosis.