[2P-176] Examination of the amino acid residues necessary for the substrate binding of yeast 14-demethylase(CYP51)

^○Masashi Miki¹, Yuzo Yoshida², Yuri Aoyama^1,3 (1.Grad. Sch. of Engineering, Soka University, 2.Sch. of Pharm. Science, Mukogawa Women's University, 3.Fac. of Science and Engineering, Soka University)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 2:45 PM - 3:45 PM

[2P-177] A new aspect of molecular evolution of indoleamine 2,3-dioxygenase.Season 8: Two amino acid substitutions are sufficient to bestow high affinity for L-Trp on IDO2.

^○Hajime Yuasa (Fac. of Sci., Kochi Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 1:30 PM - 2:45 PM

[2P-178] Purification and characterization of moss kaurene oxidase (CYP701B1) expressed in Pichia yeast

^○Chisato Noguchi¹, Sho Miyazaki², Hiroshi Kawaide³, Yuri Aoyama^1,4 (1.Grad. Sch. of Engineering, Soka University, 2.Grad.Sch. of Agricultural and Life Science, the University of Tokyo, 3.Dep. of Applied Biological Science, Fac. of Agriculture, Tokyo University of Agriculture and Technology, 4.Fac. of Science and Engineering, Soka University)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 2:45 PM - 3:45 PM

[2P-179(2T12-07)] Phenotype and vitamin D metabolites analysis of CYP27B1 knockout rats generated by CPISPR/Cas9- mediated genome editing

^○Miyu Nishikawa^1,2, Kaori Yasuda¹, Hiroki Mano¹, Masashi Takamatsu¹, Keisuke Abe¹, Shinichi Ikushiro¹, Toshiyuki Sakaki¹ (1.Dep. of Biotech., Toyama Pref. Univ., 2.TOPUBIO Research Co., Ltd.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 1:30 PM - 2:45 PM

[2P-180(2T12-05)] Genetic and biochemical characterization of SufBCD complex involved in biosynthesis of iron-sulfur clusters

^○Eiki Yuda¹, Takashi Fujishiro¹, Naoyuki Tanaka¹, Kei Hirabayashi^2,3, Keiichi Fukuyama⁴, Kei Wada², Yasuhiro Takahashi¹ (1.Div. of Life Sci., Grad. Sch. of Sci. and Eng., Saitama Univ., 2.Org. for Promotion of TT, Univ. of Miyazaki, 3.Dept. of Appl. Biol. Chem., Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 4.Div. of Appl. Chem., Grad. Sch. of Eng., Osaka Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 2:45 PM - 3:45 PM

[2P-181(2T12-06)] Haem-dependent dimerization ofPGRMC1/Sigma-2 receptor facilitatescancer proliferation and chemoresistance

^○Yasuaki Kabe, Makoto Suematsu (Dept. of Biochemistry, School of Medicine, Keio Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 1:30 PM - 2:45 PM

[2P-182] Detection of trypsin-like protease in tissue using biotin-labeled substrate

^○Hiroyuki Yamamoto, Yuki Toyota, Naoyuki Wakamatsu, Haruko Yamaguchi, Michio Kimura (Depart. Microbiol. Mol. Cell Biol., Nihon Pharm. Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 2:45 PM - 3:45 PM

[2P-183] Genetic analysis and expression of nucleoside diphosphate kinase gene from Anoxybacillus flavithermus

^○Kouta Nagai¹, Daiki Fujimoto¹, Narumi Maegawa¹, Kenji Akiyoshi-Hiraoka², Shota Inoue², Yurie Ohiwa², Takanori Satoh³ (1.Biochem.Lab., Fac. of IAS, Tokushima Univ., 2.Biochem.Lab., Grad.Sch.of IAS, Tokushima Univ., 3.Biochem.Lab., Grad. Sch. of Sci. Tech., Tokushima Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 1:30 PM - 2:45 PM

[2P-184] Identification of lipopolysaccharide-binding site of factor B in horseshoe crabs

^○Toshiaki Takahashi¹, Yuki Kobayashi^2,3, Toshio Shibata^2,4, Hikaru Mizumura³, Toshio Oda³, Shun-ichiro Kawabata^1,2 (1.Grad. Sch. of Sys. Life. Sci., Kyushu Univ., 2.Dept. of Bio., Fac. of Sci., Kyushu Univ., 3.LAL Res. and Dev. Gr., Seikagaku Corp., 4.Inst. for Adv. Study, Kyushu Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 2:45 PM - 3:45 PM

[2P-185] Genetic analysis and expression of uridine kinase gene from Anoxybacillus flavithermus

^○Shota Inoue¹, Kouta Nagai², Daiki Fujimoto², Narumi Maegawa², Kenji Akiyoshi-Hiraoka¹, Yurie Ohiwa¹, Takanori Satoh³ (1.Biochem.Lab., Grad.Sch.of IAS, Tokushima Univ., 2.Biochem.Lab., Fac. of IAS, Tokushima Univ., 3.Biochem.Lab., Grad. Sch. of Sci. Tech., Tokushima Univ.)

The 89th Annual Meeting of JBS |Mon. Sep 26, 2016 1:30 PM - 2:45 PM