[1P-144(1T06a-07)] Development and functional assessment of an oxidative folding catalyst regurated by Ca²⁺

^〇Rumi Mikami^1,2, Shingo Kanemura^3,4, Masaki Okumura³, Kenta Arai^1,2 (1.Department of Chemistry, School of Science, Tokai University, 2.Institute of Advanced Biosciences, Tokai University, 3.Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 4.Department of Chemistry, School of Science, Kwansei Gakuin University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-145(1T06a-08)] Development of oligonucleotide-based PROTACs for expanding the applicability of targeted protein degradation

^〇Miyako Naganuma^1,2, Nobumichi Ohoka², Genichiro Tsuji², Mikihiko Naito³, Takao Inoue², Yosuke Demiz^1,2,4 (1.Graduate School of Medical Life Science, Yokohama City University, 2.National Institute of Health Sciences, 3.Graduate School of Pharmaceutical Sciences, The University of Tokyo, 4.Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-146] Mechanism of p53 degradation through the cooperation of nuclear UFD pathway E3 ligase and MDM2

^〇Takahiro Yanagawa¹, Shoshiro Hirayama¹, Anri Nishizawa¹, Hidetake Kosako², Shigeo Murata¹ (1.Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2.Fujii Memorial Institute of Medical Sciences, Tokushima University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-147] ESCRT-I complex is involved in the proteasomal degradation of cholesterol synthase on the endoplasmic reticulum.

^〇Seito Matsui¹, Yusen Men¹, Shoshiro Hirayama¹, Hidetaka Kosako², Shigeo Murata¹ (1.Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2.Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-148] Mechanistic aspects of PROTAC-mediated ubiquitination and p97 requirement

^〇Kouta Nakadai^1,2,3, Akinori Endo³, Takuya Tomita³, Norihito Shibata⁴, Yousuke Demizu^3,5,6, Kousuke Aritake⁷, Yasushi Saeki² (1.Graduate School of Frontier Sciences, The University of Tokyo, 2.Division of Protein Metabolism, Institute of Medical Science, The University of Tokyo, 3.Laboratory of Protein Metabolism,Tokyo Metropolitan Institute of Medical Science, 4.Division of Biochemistry, National Institute of Health Sciences, 5.Division of Organic Chemistry, National Institute of Health Sciences, 6.Graduate School of Medical Life Science, Yokohama City University, 7.Laboratory of Chemical Pharmacology, Daiichi University of Pharmacy)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-149] Analysis of DSB repair-coupled proteasomal degradation mechanism

^〇Maiko Okada¹, Ai Horio¹, Zhong Heng Cheah¹, Keiji Suzuki², Ryotaro Nishi¹, Kazuyoshi Yano¹ (1.School of Bioscience and Biotechnology, Tokyo University of Technology, 2.Atomic Bomb Disease Institute, Nagasaki University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-150] The ubiquitin ligase RNF181 plays a pivotal role in sustaining the mammalian proteasome activity under proteasome impairment

^〇Jun Kishiki¹, Eiichi Hashimoto¹, Shoshiro Hirayama¹, Hidetaka Kosako², Jun Hamazaki¹, Shigeo Murata¹ (1.Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan., 2.Division of Cell Signaling, Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, Japan.)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-151] Identification of a novel transcription factor of the mammalian proteasome

^〇Sota Hashimoto¹, Yan Wang¹, Yaojia Dong¹, Shoshiro Hirayama¹, Jun Hamazaki¹, Shigeo Murata¹ (Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-152] Exploring mammalian proteasome regulators by Genome-wide CRISPRi screening

^〇Maki Inami¹, Akihiro Kawabe¹, Shoshiro Hirayama¹, Jun Hamazaki¹, Shigeo Murata¹ (Graduate School of Pharmaceutical Sciences, The University of Tokyo)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-153] The 26S proteasome localizes to unique nuclear foci in senescent cells

^〇Hiroaki Iio¹, Masakazu Kato², Hidetaka Kosako³, Yasushi Saeki⁴, Jun Hamazaki¹, Murata Shigeo¹ (1.Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, the University of Tokyo, 2.Faculty of Pharmaceutical Sciences, Teikyo Heisei University, 3.Division of Cell Signaling, Fuji Memorial Institute of Medical Sciences, Tokushima University, 4.Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science)

The 96th Annual Meeting of the Japanese Biochemical Society