[XIV-29-04_06] 畜産物利用（XIV-午前）

[XIV29-05E] Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens

^○高木 理宏^1,2, 小松 稜弥^1,2,3, Julio Villena⁴, Leonardo Albarracin⁴, Md. Aminul Islam^1,2, 須田 義人⁵, 大坪 和香子^1,2,3, 野地 智法^1,2,3, 麻生 久^1,2,3, 北澤 春樹^1,2,3 (1.東北大院農, 2.食と農免疫国際教育研究セ, 3.日本学術振興会研究拠点形成事業, 4.アルゼンチン国立乳酸菌研, 5.宮城大)

[Objective] Bovine mastitis is a one of most economically devastating diseases in dairy cattle, resulting from a complex host-pathogen interactions. This study was to identify the transcriptome alterations associated with innate immune responses to mastitis caused by E. coli and S. aureus infection.
[Methods] Bovine mammary epithelial cells were stimulated with either LPS or two strains of S. aureus for 12 h and the transcriptome profiles were generated by using the Bovine (V2) Gene Expression Microarray. Selected deregulated genes including toll-like receptors 1-9 (TLR 1-9) were quantified by RT-qPCR .
[Results] A total of 429, 520 and 465 genes were differentially expressed after LPS, S. aureus strains stimulation, respectively. Expression of TLR 1-9 were confirmed. LPS-induced up regulated genes are found to be related to immune responses. Identified genes could be useful for evaluation of immunobiotics for mastitis control.