[2Np] Hygiene, Sterilization, Antibacterial activity

[2Np-11] Development of rapid detection technique for microorganisms using artificial siderophore-modified electrodes

*Inomata Tomohiko¹, Endo Suguru¹, Ido Hiroki¹, Miyamoto Masakazu¹, Ichikawa Hiroki¹, Sugita Ririka¹, Ozawa Tomohiro¹, Masuda Hideki² (1. Nagoya Institute of Technology, 2. Aichi Institute of Technology)

Keywords:artificial siderophore, detection of microorganisms, modified electrodes

Objective:
Siderophores. Artificial siderophores are artificial molecules that mimic the function of natural siderophores which are chelating molecules produced by microorganisms for Fe ions uptake. In this study, we report a technique for rapid and selective detection of microorganisms using electrodes modified with artificial siderophore molecules with different structures.

Methods:
Artificial siderophore molecules of catecholate-type, hydroxamate-type, and hybrid types containing both were designed and synthesized as iron ion capturing sites, and were modified on the electrode surface.

Results:
The adsorption behavior of various microorganisms on the artificial siderophore-modified electrodes with different structures was observed, and it was found that microorganisms were easily adsorbed on the artificial siderophore-modified electrodes which have similar structures to the natural siderophores. We developed the rapid and selective microorganism detection techenique using this phenomenon.