[3Ep] Regulating effect on gastrointestinal function, Lipid metabolism regulation, Colesterol regulation

[3Ep-03] Synbiotic effects of baker's yeast (Saccharomyces cerevisiae) cell walls and W.coagulans SANK 70258 -in vitro human stool culture system-

*Motonori Miyago¹, Mutsuki Takahashi¹, Misaki Hatanaka¹, Motoyuki Tagashira¹, Yuta Noda², Akiko Sakamoto², Ryoichi Yamada² (1. ASAHI GROUP FOODS,LTD. , 2. Mitsubishi Chemical Corporation)

Keywords:Synbiotic, yeast cell wall, spore-forming lactic acid bacterium, short chain fatty acid, flora

[Purpose] Yeast cell wall(YCW) refers to the outermost part of yeast that covers the cell. It contains β-glucan and mannan. On the other hand, W.coagulans SANK 70258(COA) is a spore-forming lactic acid bacterium and has been reported to improve the intestinal environment. In this report, we report the results of synbiotic effects of baker's YCW and COA.
[Method] Feces collected from 13 Japanese people were suspended in a medium. The suspension was mixed with each of 4 samples(① No addition (CON) ②COA ③YCW ④COA+YCW(SYN)) and was cultured anaerobically. After culturing, 16S rRNA flora and short chain fatty acid were measured.
[Results] Butyrate showed significantly higher values in the SYN addition group compared to CON and YCW. The reason was thought to be that butyrate production was activated using lactic acid produced by COA and acetic acid produced by YCW. In addition, the SYN addition group had a growth promoting effect on Bacteroides, and Faecalibacterium. In conclusion, it was found that YCW and COA effectively increased butyrate by complementarily promoting the butyrate production pathway.