[2P-249] Isomeric Analysis of Oligosaccharide and Glycoside Derivatives using Borate Complexes and Mass Spectrometry

^○Kurogochi Masaki, Mizuno Mamoru, Matsuda Akio (The Noguchi Institute)

The 91th Annual Meeting of JBS

[2P-250] Binding analysis of ERK2 and EBITEIN1 using surface plasmon resonance (SPR) spectroscopy -continued report-

^○Miura Kenji (Dept. Devel. Anat. Regen. Biol. / Central Research Labo., Nation. Defense Med. Col.)

The 91th Annual Meeting of JBS

[2P-251] Label-free quantification of proteome using shotgun nLC-MS/MS

^○Dohmae Naoshi, Suzuki Takehiro (Biomolecular Characterization Unit, RIKEN CSRS)

The 91th Annual Meeting of JBS

[2P-252(2T14m-01)] TAMRA-Phos-tag fluorescent gel staining of phosphorylated proteins

^○Kusamoto Hiroshi, Nishimura Tomoyo, Kinoshita-Kikuta Emiko, Kinoshita Eiji, Koike Tohru (Graduate School of Biomedical & Health Sciencs, Hiroshima University)

The 91th Annual Meeting of JBS

[2P-253] Fluorescence correlation spectroscopy analysis of RNA degradation in cells

^○Shimada Naotaka¹, Watanabe Kazunori¹, Ohtsuki Takashi^1,2 (1.Graduate School of Natural Science and Technology, Okayama University, 2.Graduate School of Interdisciplinary Science and Engineering in Health Systems)

The 91th Annual Meeting of JBS

[2P-254] pH imaging in living prokaryotic cells and organelles using pH-sensitive fluorescent protein

^○Eguchi Yukako¹, Taoka Azuma¹, Fukumori Yoshihiro² (1.Sch. of Biosci. and Tech., Col. of Sci. and Eng., Kanazawa Univ., 2.Vice President,Trustee, Kanazawa Univ.)

The 91th Annual Meeting of JBS

[2P-255(2T14m-02)] Development of Novel Disease Diagnosis Platform based on Enzyme Activity Detection at Single Protein Level

^○Sakamoto Shingo¹, Komatsu Toru^1,5, Watanabe Rikiya^4,5, Yi Zhang⁴, Noji Hiroyuki⁴, Urano Yasuteru^1,2,3 (1.Grad. Sch. Pharm. Sci., The Univ. Tokyo, 2.Grad. Sch. Med., The Univ. Tokyo, 3.AMED CREST, 4.Grad. Sch. Eng., The Univ. Tokyo, 5.JST PRESTO)

The 91th Annual Meeting of JBS

[2P-256(2T14m-03)] Single cell analysis of necroptosis execution by FRET biosensor

^○Murai Shin¹, Yamaguchi Yoshifumi², Shirasaki Yoshitaka³, Shindo Ryodai¹, Nakabayashi Osamu¹, Joanne Hildebrand⁴, Tomida Taichiro⁵, Akahane Satomi⁵, John Silke⁴, Miura Masayuki⁶, Nkano Hiroyasu^1,7 (1.Division of Medical Chemistry, Department of Biochemistry, Faculty of Medicine, Toho University, 2.Hibernation Metabolism, Physiology, and Development Group, ILTS, Hokkaido University, 3.Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 4.WEHI, University of Melbourne, 5.Department of Physiology, School of Medicine, Faculty of Medicine, Toho University, 6.Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7.Toho University Host Defence Reaseach Center)

The 91th Annual Meeting of JBS

[2P-257] Evaluation of calcium ion permeability using GUVs prepared by a droplet transfer method

Uno Yuki, ^○Nishigami Misako, Tomita Masahiro, Tsumoto Kanta (Grad. Sch. of Eng., Mie Univ.)

The 91th Annual Meeting of JBS

[2P-258(2T14m-04)] Isotopomer tracing approach to investigate pathways for complete glucose synthesis from methanol through a model network of carbohydrate metabolism including reactions for methanol utilization

^○Ohta Jun (Okayama University)

The 91th Annual Meeting of JBS