[A25-2am] 17. Biofunctional Chemistry, Biotechnology

[A25-2am-07] Nucleic Acids Chemistry beyond the Watson-Crick Double Helix (72): Prediction of RNA/DNA hybrid stability under a physiological condition and verification of advantage in CRISPR-Cas9

^○Dipanwita Banerjee¹, Hisae Tateishi-Karimata¹, Tatsuya Ohyama¹, Marko Trajkovski², Maria Toplishek², Janez Plavec^2,3,4, Naoki Sugimoto^1,5 (1. FIBER, Konan University, 2. Slovenian NMR Centre, National Institute of Chemistry, 3. EN→FIST Centre of Excellence, 4. Faculty of Chemistry and Chemical Technology, University of Ljubljana, 5. FIRST, Konan University)

[Lang.] English

Keywords:RNA/DNA hybrid duplex, Thermodynamic stability, Physiological condition, Nearest-neighbor parameters, CRISPR-Cas9 technique