[PA-02] Investigation of a method for measuring the amount of glycoalkaloids in potatoes using near-infrared spectroscopy.
Objective
Potatoes are a food crop that is widely grown and consumed throughout the world. Glycoalkaloids (solanine and caconine) contained in potatoes cause poisoning symptoms when their intake exceeds a certain amount. In this study, we investigated the application of near-infrared spectroscopy as a rapid and simple method for the determination of glycoalkaloids.
Methods
Specimens were selected from the variety "Sayaka" and were irradiated with green light for 4 days to increase the glycoalkaloid content, and those stored in the dark were used. The glycoalkaloid content after each irradiation was calculated from a calibration curve using the specimens. The correlation between the concentration obtained and the absorbance spectra obtained from near-infrared irradiation (900-1700 nm) was analyzed.
Results
The correlation between the near-infrared absorption spectrum and the amount of glycoalkaloids was analyzed. As a result, specific absorption peaks were observed in the wavelength regions of 900-940 nm and 1070-1130 nm. These absorption wavelengths were consistent with the absorption of ArCH groups derived from the steroidal skeleton of caconin.
Potatoes are a food crop that is widely grown and consumed throughout the world. Glycoalkaloids (solanine and caconine) contained in potatoes cause poisoning symptoms when their intake exceeds a certain amount. In this study, we investigated the application of near-infrared spectroscopy as a rapid and simple method for the determination of glycoalkaloids.
Methods
Specimens were selected from the variety "Sayaka" and were irradiated with green light for 4 days to increase the glycoalkaloid content, and those stored in the dark were used. The glycoalkaloid content after each irradiation was calculated from a calibration curve using the specimens. The correlation between the concentration obtained and the absorbance spectra obtained from near-infrared irradiation (900-1700 nm) was analyzed.
Results
The correlation between the near-infrared absorption spectrum and the amount of glycoalkaloids was analyzed. As a result, specific absorption peaks were observed in the wavelength regions of 900-940 nm and 1070-1130 nm. These absorption wavelengths were consistent with the absorption of ArCH groups derived from the steroidal skeleton of caconin.