[P-265(2T15m-11)] Development of protein degrader-dependent interactome technology using a novel proximity-dependent biotinylation enzyme AirID

^○Satoshi Yamanaka¹, Yuto Horiuchi¹, Kohei Nishino², Hidetaka Kosako², Tatsuya Sawasaki¹ (1.Division of Cell-Free Sciences, Proteo-Science Center, Ehime University, 2.Institute of Advanced Medical Sciences, Fujii Memorial Institute of Medical Sciences, Tokushima University)

The 94th Annual Meeting of JBS

[P-266(2T15m-12)] Development of a selective degrader targeting hematopoietic prostaglandin D synthase

^○Norihito Shibata¹, Hidetomo Yokoo², Akinori Endo³, Takahito Ito^4,5, Yuta Yanase^4,5, Yuki Murakami^4,5, Kiyonaga Fujii⁶, Kazunari Kondo¹, Yasushi Saeki³, Mikihiko Naito⁷, Kosuke Aritake⁸, Yosuke Demizu^4,5 (1.Division of Biochemistry, NIHS, 2.Medical Chemistry, Kyoto Prefectural University of Medicine, 3.Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science, 4.Division of Organic Chemistry, NIHS, 5.Graduate School of Medical Life Science, Yokohama City University, 6.Laboratory of Analytical Chemistry, Daiichi University of Pharmacy, 7.Laboratory of Targeted Protein Degradation, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 8.Laboratory of Chemical Pharmacology, Daiichi University of Pharmacy)

The 94th Annual Meeting of JBS

[P-267(2T15m-08)] Genome-wide CRISPR/Cas9 screening for the lysosomal degradation of misfolded membrane proteins at the ER

^○Satoshi Hayakawa, Yuki Hayashi, Hidenori Ichijo (Laboratory of Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo)

The 94th Annual Meeting of JBS

[P-268(2T15m-09)] A novel membrane protein quality control system at the endoplasmic reticulum mediated by selective lysosomal degradation

^○Yuki Hayashi, Hidenori Ichijo (Laboratory of Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo)

The 94th Annual Meeting of JBS

[P-269(2T15m-07)] Degradation mechanisms of NRF1: a transcription factor of proteasomal genes

^○Daiki Kinoshita¹, Yukiko Yoshida¹, Noriyuki Matsuda¹, Keiji Tanaka² (1.Ubiquitin Project, Tokyo Metropolitan Institute of Medical Science, 2.Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science)

The 94th Annual Meeting of JBS

[P-270] Elucidation of the regulatory mechanism of exosome secretion via BAG6

^○Jun Shirai, Toshiki Takahashi, Hiroyuki Kawahara (Lab. Cell. Biochem., Dept. of Biol. Sci., Grad. Sch. of Sci., Tokyo Metropolitan Univ.)

The 94th Annual Meeting of JBS

[P-271] Development of anti-MSP monoclonal antibodies for nanodisc-based application

^○Fuhito Nakagawa, Sisi Chin, Satoshi Yasuda, Takeshi Murata, Satoshi Ogasawara (Graduate school of Science, Chiba University)

The 94th Annual Meeting of JBS

[P-272(3T14m-12)] Search for biomarkers useful for differential diagnosis of renal diseases by urinary and plasma proteomics.

^○Tsukasa Osaki^1,2, Akira Araumi³, Kazuma Ichikawa³, Masafumi Watanabe³, Tsuneo Konta² (1.Department of Biochemistry and Molecular Biology, Yamagata University Faculty of Medicine, Yamagata, Japan, 2.Department of Public Health and Hygiene, Yamagata University Faculty of Medicine, Yamagata, Japan, 3.Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan)

The 94th Annual Meeting of JBS

[P-273] Diverse subcellular localization of Bcnt/Cfdp1, a disordered Hub protein, in both growing and differentiated myotube of C2C12 cells

^○Shintaro Iwashita^1,4, Takehiro Suzuki², Takeshi Yasuda³, Yoshimitsu Kiriyama¹, Yoshiharu Ohoka¹, Kentaro Nakashima^1,4 (1.Kagawa Sch. Pharm. Sci., Tokushima Bunri Univ., 2.Biomol. Characterization Unit, CSRS, RIKEN, 3.iQLS, QST, 4.Inst. of Neurosci.)

The 94th Annual Meeting of JBS

[P-274] Evaluation of antigen recognition mechanism using single-chain Fv antibody G2 that recognizes multiple epitopes

^○Takahiro Hayashi¹, Yuji O. Kamatari², Masayuki Oda¹ (1.Grad. Sch. Life Environ. Sci., Kyoto Pref. Univ., 2.Life Sci. Res. Center, Gifu Univ.)

The 94th Annual Meeting of JBS