[2P-0147] Anti-EGFR VHH Antibody under Thermal Stress Is Better Solubilized with a Lysine than with an Arginine SEP Tag

^○Akari Fukutani¹, Md.Golam Kibria¹, Yoko AkazawaOgawa², Yoshihisa Hagihara², Yutaka Kuroda¹ (1.Dept. of Biotech. and Life Sci., Grad. Sch. of Eng., TUAT, 2.AIST)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0148] Reverse engineering of high-temperature reversible oligomers and amyloids in PDZ3

^○Sawaros Onchaiya¹, Tomonori Saotome^{2, 3}, Shunichi Kidokoro³, Jose Martinez⁴, Yutaka Kuroda¹ (1.Dept. of Biotech. and Life Sci., TUAT, 2-24-16, Naka-cho, Koganei-shi, Tokyo, 184-8588, Japan., 2.Inst. of GIR, TUAT, 3-8-1, Harumi-cho, Fuchu-shi, Tokyo, 183-8538, Japan., 3.Dept. of Bio.eng, Nagaoka Gidai, Niigata, 940-2188, Japan., 4.Dept. of Phy. Chem. and Inst. of Biotech., Fac. of Sci., Univ. of Granada, 18071, Granada, Spain.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0149] In vivo immunogenicity of amorphous aggregates made of misfolded anti-EGFR single domain antibody (VHH)

^○MD. KIBRIA¹, Yoko AkazawaOgawa², Yoshihisa Hagihara², Yutaka kuroda¹ (1.Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24- 16 Nakamachi, Koganei-shi, Tokyo, 184-8588, Japan, 2.Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Osaka, 563-8577, Japan)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0150] Expression of two disulfide-bonded EGFR extracellular domain III in Escherichia coli using a SEP tag: Biophysical, functional characterization, and its immunogenic potential.

^○Brindha Subbaian, Md. Golam Kibria, Yutaka Kuroda (Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei-shi, Tokyo 184-8588, Japan)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0151] Molecular size difference of Entamoeba histolytica Igl1 lectin in different culture conditions

^○Kentaro Kato^{1, 2}, Hisatoshi Hanamatsu³, Junichi Furukawa³, Tomomitsu Hatakeyama⁴, Hiroshi Tachibana⁵ (1.Dept. Eco-epidemiol., NEKKEN, Nagasaki Univ., 2.TMGH, Nagasaki Univ., 3.Dept. Adv. Clin. Glycobiol., Hokkaido Univ., 4.Biomol. Chem. Lab., Grad. Sch. Eng., Nagasaki Univ., 5.Dept. Infect. Dis., Tokai Univ. Sch. Med.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0152] Mechanism of structural change of HspB1 from CHO cells

^○Niina Kurokawa¹, Manami Nakamura¹, Ken Morishima², Rintaro Inoue², Masaaki Sugiyama², Masafumi Yohda¹ (1.Dept. of Biotechnol. Tokyo Univ. of Agric. and Technol., 2.Institute for Integrated Radiation and Nuclear Science, Kyoto Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0153] Elucidating the connection between apoptosis and autophagy in osteosarcoma mediated by ACA-28, a novel anti-cancer compound

^○Sae Kamiyama¹, Teruaki Takasaki², Nanami Ueno², Ryosuke Satoh², Toshihiro Akisue³, Reiko Sugiura^{1, 2} (1.Grad. sch. of Pharm., Kindai Univ., 2.Fac. of pharm., Kindai Univ., 3.Grad. Sch. of Health Sci., Kobe Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0154] Analysis of the effect of shedding on phosphorylation of Rab7

^○Yurika Aoi, Fumiya Sakamoto, Kyoko Shirakabe (Univ. of Ritsumeikan)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0155] Regulatory mechanism of cell functions through ectodomain shedding of ALCAM adhesion molecule

^○Tomoyo Takagi, Mayu Matsuoka, Kyoko Shirakabe (Univ. of Ritsumeikan)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[2P-0156] Insulin amyloid fibrils interact directly with the NLRP3, resulting in inflammasome activation and pyroptotic cell death

^○Wakako Mori¹, Naoe Kaneko^{2, 3}, Ayaka Nakanishi¹, Tamotsu Zako¹, Junya Masumoto^{2, 3} (1.Analytical Chemistry, Graduate School of Science and Engineering, Ehime University., 2.Analytical Pathology, Graduate School of Medicine, Ehime University., 3.Pathology, Proteo-Science center, Ehime University.)

The 44th Annual Meeting of the Molecular Biology Society of Japan