[P3-19] Expression and Function of Chemokines in Bovine Endometrium and Embryos
Chemokines, which mainly regulate immune cells, also involve the cellular remodeling in endometrium of human and rodents during pregnancy. However, the expression and function of chemokines in the bovine uterus remains still unclear. The purpose of this study is to analyze the expression pattern and functional properties of chemokines in the bovine endometrium and embryos. The qPCR analysis of the tissue showed that the amount of CCL2, CCL8 and CXCL10 transcripts were high at the implantation stage. The amount of CCL2 transcripts was significantly high in IFNa treated bovine endometrial stromal (BES) cells in vitro. In bovine endometrial epithelial (BEE) cells, however, the amount of CCL8 and CXCL10 were significantly high in the treatment group, but not for CCL2. The mRNA of each chemokine receptors was detected in the endometrial tissues and embryos by RT-PCR. Cellular proliferation of BEE and BES significantly increased by the CCL2 treatment. These results indicate that the expression of chemokines increased in endometrium during implantation and possible to regulate the proliferation of both endometrium and embryos.