[3Ba] Physiologically active compounds

[3Ba-05] Verification of a non-destructive measurement method for β-cryptoxanthin in mid-late ripening citrus cultivars using visible and near-infrared spectroscopy

*MINORU SUGIURA^1,2, AYAMI HISANAGA², TATSUYUKI SAKANAKA³, TERUTAKA YOSHIOKA⁴ (1. Doshisha Women’s College of Liberal Arts, 2. Division of Citrus Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 3. Saika Technological Institute Foundation, 4. Kyushu-Okinawa Agricultural Research Center, National Agriculture and Food Research Organization (NARO))

Keywords:non-destructive measurement, visible and near-infrared spectroscopy, mid-late ripening citrus cultivars, carotenoids, beta-cryptoxanthin

Previously, we have found that the use of visible and near-infrared (VIS-NIR) spectroscopy is a useful nondestructive method for quantification of β-cryptoxanthin in Satsuma mandarin as an alternative method to HPLC. In this study, we examined whether the use of VIS-NIR spectroscopy would permit the quantification of βcryptoxanthin in mid-late ripening citrus cultivars. Examination of the associations between VIS-NIR spectra data and β-cryptoxanthin contents was performed using partial least squares regression (PLSR). We found that the absorption in the region of 600 nm is particularly important for estimating the β-cryptoxanthin content, and that the wavelength regions related to sugar content also affect the assessment of β-cryptoxanthin. From these results, it is suggested that the use of VIS-NIR spectroscopy could allow prediction of β-cryptoxanthin content in mid-late ripening citrus cultivars.