優秀発表４

[IVYS-06] Lipopolysaccharides infusion into the uterus causes inflammation in the mammary gland

〇Purba Fika¹、新居 隆浩²、吉村 幸則²、磯部 直樹² (1.大学院生物圏科学研究科、2.大学院統合生命科学研究科)

This study was carried out to demonstrate whether intrauterine-infused lipopolysaccharide (LPS) was translocated to the mammary gland, resulting in its inflammation. In Experiment 1, sixteen goats were intrauterine-infused with saline or LPS, while in Experiment 2, dexamethasone was administered before the infusion. Milk and blood were collected to measure the cytokines, acute-phase proteins, and antimicrobial peptides. In Experiment 1, the concentrations of IL-1β and IL-6 in milk were higher in the LPS group. In Experiment 2, somatic cell count was higher, whereas the milk yield and blood leukocyte count were lower in the LPS group than that in the control group. Lipopolysaccharide-binding protein and serum amyloid A in plasma, IL-1β, S100A8, and lactoferrin in milk were significantly higher in the LPS group. Taken together, these results suggest that LPS can be translocated from the uterus to the mammary glands and cause inflammation, especially under the immunosuppressive condition.