[P1(1F)] Poster Session 01(4F-1) Poster Award candidate

[P1-1F-149] High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber

*Minh Nguyen Tuyet Le¹, Shota Tashiro¹, Yuta Kusama¹, Eri Nakatani¹, Mika Suga², Miho K Furue², Taku Satoh³, Shinji Sugiura³, Toshiyuki Kanamori³, Kiyoshi Ohnuma¹, Yoshikatsu Tobaru¹ (1. Nagaoka University of Technology, 2. Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan, 3. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, 5 Ibaraki 305-8562, Japan)

Keywords:Human induced pluripotent stem cells, Bone morphogenetic protein 4 (BMP4), Spatial pattern, Unidirectional perfusion culture, Cell density