[1P-0802] GGenome & CRISPRdirect: web servers for designing CRISPR/Cas9 guide RNA

〇Yuki Naito¹, Hidemasa Bono¹ (1.Database Center for Life Science (DBCLS))

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0803] Discovery and characterization of novel genome regions for gene knock-in.

〇Isamu Nanchi^1,2, Yuki Yoshimura^2,4, Kazuomi Nakamura³, Yusaku Masago¹, Tetsuya Obayashi³, Tomohiko Okuda¹ (1.Drug Disc. & Dis. Res. Lab., SHIONOGI & CO., LTD., 2.Inst. of Regen. Med. and Biof., Grad. Sch. of Med. Sci., Tottori Univ., 3.Res. Cent. for Bios. and Tech., Tottori Univ., 4.CIEA)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0804] Precise genome editing at single-base resolution

〇Atsushi Satomura^1,2, Kouichi Kuroda¹, Mitsuyoshi Ueda¹ (1.Div. Appl. Life Sci., Grad. Sch. Agric., Kyoto Univ., 2.Japan Society for the Promotion of Science)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0805] Generation of knockout cell lines by efficient delivery of CRISPR/Cas9 for genome editing.

〇Ken Inoue¹, Yukari Inoue¹, Kiyokazu Kemmochi¹, Yasutomo Kisu¹, Rinichi Yamashita¹ (1.Thermo Fisher Scientific Life Technologies Japan Ltd.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0806] Activation of MMEJ repair pathway enhances gene knock-in in human cells.

〇Shota Nakade¹, Tetsushi Sakuma¹, Keiji Mochida¹, Ayu Oishi¹, Takashi Yamamoto¹ (1.Dept. of Math. and Life Sci., Grad. Sch. of Sci., Hiroshima Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0807(3AS6-2)] Versatile protein tagging with split fluorescence protein: a scalable strategy for GFP tagging of endogenous human proteins

〇Sayaka Sekine^1,3, Daichi Kamiyama^1,4, Manuel Leonetti², Jonathan Weissman², Bo Huang¹ (1.Dept. Pharm Chem., UCSF, 2.Dept. Cell. Mol. Pharm., UCSF, 3.RIKEN CDB, 4.Dept. Cell. Biol., Univ. of Georgia)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0808] Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting

〇Junko Masuda¹, Hiroshi Kawamoto², Eiji Takayama³, Akifumi Mizutani¹, Hiroshi Murakami¹, Tomokatsu Ikawa⁴, Narumi Maeno¹, Tsukasa Shigehiro¹, Ayano Satoh¹, Akimasa Seno¹, Arun Vaidyanath¹, Tomonari Kasai¹, Yoshimoto Katsura⁵, Masaharu Seno¹ (1.Grad. Sch. of Nat. Sci. & Tech., Okayama Univ., 2.Dept. of Immun, Inst. for Front. Med. Sci., Kyoto Univ., 3.Dept. of Oral Biochem., Asahi Univ. Sch. of Dent., 4.Lab. for Immune Regen., RIKEN Cent. for Integ. Med. Sci., 5.Dev. of Cell Regen. & Transpl. Med,, Nihon Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0809] Monitoring of the diversification of the ADLib system libraries

〇Akiho Murayama¹, Hidetaka Seo¹, Kunihiro Ohta¹ (1.Dept. of Life Sci., Grad. Sch. of Art & Sci., Univ. of Tokyo)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0810] Development of highly functional antibodies using B cell-derived culture cells

〇Koji Hashimoto¹, Kohei Kurosawa², Akiho Murayama¹, Hidetaka Seo¹, Kunihiro Ohta¹ (1.Dept. of Life Sci., Grad. Sch. of Arts and Sci., Univ. of Toky, 2.NYU Langone Medical Center)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[1P-0811] Fine tuning of antibody specificity by mutations focused on framework residues

〇Junichi Nakayama¹, Takamitsu Hattori¹, Hikaru Nakazawa¹, Mika K. Kaneko², Yukinari Kato², Izumi Kumagai¹, Mitsuo Umetsu¹ (1.Dept. of Biomol. Eng.,Grad. Sch. of Eng., Univ. of Tohoku, 2.Dept. of Reg. Innov.,Grad. Sch. of Med., Univ. of Tohoku)

The 39th Annual Meeting of the Molecular Biology Society of Japan