[3P-0831(3AS6-4)] Efficient genome editing of iPS cells with a piggyBac-based dual-regulated CRISPR-Cas9 system for dissecting the molecular mechanism of human diseases

〇Kentaro Ishida¹, Noriko Sasakawa¹, Julia Kudryashev², Peter D. Gee¹, Huaigeng Xu¹, Akitsu Hotta¹ (1.CiRA, Univ. of Kyoto, 2.MIT)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0832] Generation of PCS (MVA) syndrome-mutation knock-in mice using CRISPR/Cas9 system and ssODN- mediated genome editing technology

〇Kosuke Hosoba¹, Takao Tanaka², Akihiro Fukumitsu¹, Tatsuo Miyamoto¹, Shinya Matsuura¹ (1.Dept. of Genet and Cell Biol.,RIRBM, Hiroshima Univ., Japan, 2.KAC Co., Ltd., Japan)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0833] Application of genome editing to the pathophysiological analysis and the treatment of polyglutamine disease

〇Satoshi Tanaka^1,2, Takuji Ito¹, Akinobu Ota³, Takefumi Sone⁴, Daisuke Shimojo^1,4, Kazunari Onodera^1,5, Shiro Imagama², Yoshitaka Hosokawa³, Manabu Doyu¹, Masahisa Katsuno⁵, Gen Sobue⁶, Hideyuki Okano⁴, Yohei Okada¹ (1.Dept. of Neurol, Sch. of Med. Aichi Medical Univ., 2.Dept. of Orthop, Sch. of Med. Nagoya Univ., 3.Dept. of Bioc, Sch. of Med. Aichi Medical Univ., 4.Dept. of Physiol, Sch. of Med. Keio Univ., 5.Dept. of Neurol, Sch. of Med. Nagoya Univ., 6.Grad. Sch. of Med, Nagoya Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0834] Generation of iNKT Cell-deficient mice by CRISPR/hCas9 and their role in obesity.

〇Risa Shibata^1,2, Yue Ren², Susumu Nakae¹, Hiroshi Watarai^2,3 (1.Dept. of Comp. Biol. and Med. Sci., Grad. Sch. of Front. Sci., Univ. of Tokyo, 2.Div. of Stem Cell Cellomics, IMSUT, Univ. of Tokyo, 3.ImPACT, JST)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0835] Effective transfection in the cancer cell lines

〇Ken Kawamura¹, Chikako Yokoyama², Hisashi Hisatomi¹ (1.Dept. Materials and Life Sci., Seikei Univ., 2.Biochem.Eng., Grad. Sch. Sci and Eng., Yamagata Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0836(3PS8-1)] The front lines of the gene therapy using genome editing

〇Yumi Kanegae¹, Aya Maekawa², Mariko Suzuki², Izumu Saito², Saki Kondo² (1.Res.Cent. Med.Sci., Jikei Univ., 2.Mol.Gen., IMSUT, Univ. of Tokyo)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0837] Genome editing mice produced by CRISPR / Cas9 system using cryopreserved embryos and electroporation

〇Yayoi Kunihiro¹, Yoshihiro Uno¹, Kousuke Hattori¹, Tomoji Mashimo¹ (1.Inst. of Exp.Anim. Sci., Grad. Sch. of Med., Univ. of Osaka)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0838] Genome editing of embryos by electroporation

〇Kazuki Nakao^1,2, Harumi Nakao¹, Hiroshi Kiyonari², Atsu Aiba¹ (1.Lab. Ani. Res. CDBIM, Fac. Med., Univ. of Tokyo, 2.Ani. Res. Dev. Unit, CLST, RIKEN)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0839] Easi-CRISPR：Efficient method for generation of knock-in mice

〇Hiromi Miura^1,2, Gurumurthy Channabasavaiah⁵, Tomomi Aida³, Yutaka Inagaki², Masahiro Sato⁴, Takehito Sato¹, Masato Ohtsuka¹ (1.Dept. of Mol. Life Sci., Bas. Med. Sci. and Mol. Medi., Sch. of Med., Tokai Univ., 2.Dept. of Rege. Med., Bas. Med. Sci., Sch. of Med., Tokai Univ., 3.Dept. of Mol. Neuro., Med. Res. Ins. Tokyo Med. and Dent. Univ., 4.Sec. of Gene Exp. Reg., Fron. Sci. Res. Cent., Kagoshima Univ., 5.Mou. Geno. Engi. Co. Faci., Dept. of Gene. Cel. Biol. and Anat., Univ. of Nebraska Med. Cent.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[3P-0840] Generation of genome-edited mice via Cloning-free CRISPR/Cas system

〇Yukiko U. Inoue¹, Takayoshi Inoue¹ (1.Dept. of Biochem. & Cellular Biol., NCNP)

The 39th Annual Meeting of the Molecular Biology Society of Japan