[2P-0798] A system for construction of marker-free disruptants in a purple photosynthetic bacteria, Rubrivivax gelatinosus IL144 using a serine-type integrase from a Streptmyces phage, φK38-1.

〇Naoki Fusada^1,3, Hideo Takahashi³, Sakiko Nagashima^2,4, Kazuhito Inoue^1,2, Kenji V. P. Nagashima¹ (1.Res. Inst. for Photobiol. Hydrog. Production, Kanagawa Univ., 2.Dept. of Biol. Sci., Kanagawa Univ., 3.Dept. of Appl. Biol. Sci., Col. of Bioresource. Sci., Nihon Univ., 4.Dept. of Biol. Sci., Tokyo Metro. Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0799] Cyanobacterial genome arrangement using Bacillus genome vector system

Takeshi Kawaguchi¹, 〇Satoru Watanabe¹, Mitsuhiro Itaya², Hirofumi Yoshikawa¹ (1.Depr. of Biosci., Tokyo Univ., of Agric., 2.IAB, Keio Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0800(1PS11-3)] Cell Lysis Technology to provide transformable Extra-cellular DNA (CELyTED)

〇Shinya Kaneko¹, Mitsuhiro Itaya² (1.School of Life Science and Technology, Tokyo Institute of Technology, 2.Inst. Adv. Biosci. Keio Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0801] Establishment of a fast and practical transformation method of S. cerevisiae based on trans-kingdom conjugal transfer system: Just mixing the donor and recipient cultures

〇Kazuki Moriguchi¹, Shinji Yamamoto¹, Yuta Ohmine¹, Katsunori Suzuki¹ (1.Dept. of Biol. Sci., Grad. Sch. of Sci., Hiroshima Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0802] The engineered riboswitch-based inducible gene expression system for plastids in higher plants

〇Yoichi Nakahira¹, Rika Yamane¹, Atsushi Ogawa², Yuzuru Tozawa³, Takashi Shiina⁴ (1.Coll. Agr., Ibaraki Univ., 2.PROS　PROS Ctr., Ehime Univ., 3.Grad. Sch. Sci. Eng., Saitama Univ., 4.Grad. Sch. Life Environ. Sci., Kyoto Pref. Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0803] Transient sgRNA expression under controls of plant U6 promoters

〇Shinya Ideguchi¹, Atsushi Takeda¹ (1.Dept. of Life Sci., Grad. Sch., Univ. of Ritsumeikan)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0804] Reversible transgenesis system via piggyBac transposon in plants

〇Ayako Nishizawa-Yokoi¹, Seiichi Toki^1,2 (1.NARO, Inst. Agrobiol. Sci., 2.Kihara Inst. Biol. Res., Yokohama City Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0805] An attempt to creation of a SPK mutant by a genome editing technique.

〇Hikaru Matsuzaki¹, Ayane Tateshima¹, Hitomi Onodera¹, Miho Kihira¹, Tomohiro Imamura², Hiroaki Kusano¹, Hiroaki Shimada¹ (1.Dept. of Biol.&Tech., Tokyo Univ. of Sci., 2.Ishikawa Prefectural Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0806] Truncation of the calmodulin-binding domain of a rice glutamate decarboxylase (GAD1) by CRISPR/Cas9 and its application to molecular breeding

〇Kazuhito Akama¹, Masako Kanesaki¹, Masafumi Mikami^2,3, Masaki Endo², Seiichi Toki^2,3,4 (1.Dept. of Biol. Sci., Fac. of Life & Environ. Sci., Shimane Univ., 2.NARO, 3.Graduate School of Nanobiosci., Yokohama City Univ., 4.Kihara Insti., For Biol. Res., Yokohama City Univ.)

The 39th Annual Meeting of the Molecular Biology Society of Japan

[2P-0807] A trial to improve rice quality by using a novel genome editing vector and a versatile idea for high-density crop hydroponics in a plant incubator

〇Masaharu Kuroda¹, Tomoya Yamaguchi¹, Hiromoto Yamakawa¹ (1.NARO Central region. Agric. Res. Cent.)

The 39th Annual Meeting of the Molecular Biology Society of Japan