[3P-459] Development of efficient production methods of recombinant adeno-associated virus vector using human immortalized cell lines

^○Yoshitaka Miyagawa¹, Yoshiyuki Yamazaki¹, Tsutomu Igarashi^1,2,3, Taro Tomono⁴, Yuriko Sato¹, Yukage Kobari¹, Yuka Oyama¹, Motoko Yamamoto¹, Kumi Adachi¹, Yukihiko Hirai⁵, Akihiro Umezawa⁶, Takashi Okada⁵ (1.Dept. of Biochem. Mole. Biol., NIPPON MED SCH, 2.Dept. of Ophthalmol, Chiba Hokusoh Hospital NIPPON MED SCH, 3.Dept. of Ophthalmol, NIPPON MED SCH, 4.Dept. of Chem Biomol Eng, UC Berkeley, USA, 5.Div. of Mole. Medi. Genet., Univ. of TOKYO, 6.NCCHD, Dept. of Reprod Biol)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-460] Whole-cell modeling of Escherichia coli from its genome sequence

^○Kazunari Kaizu¹, Eliott Jacopin^1,2, Koichi Takahashi¹ (1.BDR, RIKEN, 2.FBS, Osaka Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-461] Effect of prolonging cell growth period by introduction of TERT transgene and application to cell-based regenerative medicine

^○Masashi Ikeno¹, Nobutaka Suzuki², Hiroyuki Sasakura¹, Kosei Takeuchi¹ (1.Dept. of Cell Biol. , Sch. of Med., Aichi Medical Univ., 2.Kazusa Genome Technologies Inc.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-462] Development of adeno-associated virus vector production methods using genome editing

^○Satsuki Anzai¹, Yoshitaka Miyagawa¹, Yukage Kobari¹, Yuriko Sato¹, Yuka Oyama¹, Mashito Sakai¹, Takashi Okada² (1.Dept. of Biochem. Mole. Biol., NIPPON MED SCH, 2.Div. of Mole. Medi. Genet., Univ. of TOKYO)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-463] A green color fluorescence lifetime-based ATP biosensor

^○Vu Cong Quang¹, Kiya Taketoshi², Fujie Toshinori³, Kitaguchi Tetsuya⁴, Arai Satoshi¹ (1.WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa, Japan, 2.Faculty of Biological Science and Technology, Institute of Science and Engineering Developmental Biology, Kanazawa University, Kanazawa, Japan, 3.School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan, 4.Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Kanagawa, Japan)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-464] Evaluation of viability/toxicity/cytokine-production of immune cells stimulated by microplastics

Tomoki Tahara¹, Yusuke Ide², Yosuke Nishimura¹, Kinichi Morita³, Shohei Nishikawa³, Shigenori Ota⁴, Yoichi Saito⁵, Yukio Fujiwara⁶, Yoshitaka Nakanishi^5,7, ^○Yuta Nakashima^5,7,8,9 (1.Dept. Mech. and Math. Eng., Fac. Eng., Kumamoto Univ., 2.Dept. Mech. and Math. Eng., Grad. Sch. Sci. and Tech., Kumamoto Univ., 3.Nishikawa KEISOKU Co., LTD, 4.GL Sciences Inc., 5.Fac. Advanced Sci. and Tech., Kumamoto Univ., 6.Fac. Life Sci., Kumamoto Univ., 7.Inst. of Industrial Nanomaterials, Kumamoto Univ., 8.Int. Res. Org. for Advanced Sci. & Tech. (IROAST), Kumamoto Univ., 9.FOREST, JST)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-465] Investigation on the essentiality of polyphosphate in Schizosaccharomyces pombe

^○Kaho Fujiyama¹, Karin Nose², Kanon Tsukuda², Kojiro Takeda^1,2 (1.Dept. of Biol., Grad. Sch. of Nat. Sci., Konan Univ., 2.Dept. of Biol., Sch. of Sci. Eng, Konan Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-466] Evaluation of extracellular Ca2+-induced permeability changes in proximal tubular epithelial tissue by trans-epithelial electrical resistance measurements

^○Yuji Takata¹, Ramin Banan Sadeghian¹, Toshikazu Araoka², Minoru Takasato^3,4,5, Kazuya Fujimoto¹, Ryuji Yokokawa¹ (1.Dept. of Eng., Grad. Sch of Eng., Kyoto Univ., 2.CiRA, Kyoto Univ., 3.BDR, 4.Grad. Sch. of Biostudies, Kyoto Univ., 5.Grad. Sch. of Med., Osaka Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-467] Simplified Protocols for generating conformation specific monoclonal antibodies suitable for biopharmaceuticals and clinical test

^○Fujino Kuba¹, Yoichiro Tanaka², Atsumi Sakaguchi³, Yasuyuki Kurihara⁴ (1.Grad. Sch. Sci. Eng., Yokohama Natl. Univ., 2.IAC, Yokohama Natl. Univ., 3.OFC, Tokyo Inst Tech, 4.Fac. Eng. Sci., Yokohama Natl. Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[3P-468] The anti-inflammatory mechanism of luteolin and luteolin-7-glucoside on macrophage differentiation

^○Fang LiWen¹, Tsai HsinJen², Chuang YuRen³ (1.Dept. of Nutr., I-Shou Univ., Kaohsiung, Taiwan., 2.Dept. of Health Manag., I-Shou Univ., Kaohsiung, Taiwan., 3.Dept. of Med. Res., E-Da Hosp., Kaohsiung, Taiwan.)

The 45th Annual Meeting of the Molecular Biology Society of Japan