[1P-068] Development of MMEJ-based long-range gene replacement with highly multiplexed CRISPR-Cas9

^○Shu Matsuzaki^1,2, Tetushi Sakuma², Takashi Yamamoto² (1.Drug Discovery Lab, Wakunaga Pharmaceutical Co., Ltd, 2.Grad. Sch. Integr. Sci. Life, Univ. of Hiroshima)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-069] Genome-resolved metagenomics reveals intra-species diversity of giant viruses

^○FANG YUE¹, MENG LINGJIE¹, XIA JUN¹, Okazaki Yusuke¹, Nagasaki Kenzo², Endo Hisashi¹, Ogata Hiroyuki¹ (1.Dept. of Biol. Sci., Grad. Sch. of Sci., Kyoto Univ., 2.Dept. of Biol. Sci., Grad. Sch. of Sci., Kochi Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-070] Mapping of a regulatory sequence of an endogenous promoter using primary Amh expressed cells.

^○Iku Tsuchiya^1,2, momoka Kasugai^1,2, Miho Terao², Shuji Takada^1,2 (1.Dept. of Systems BioMed., NCCHD, 2.Dept. of NCCHD, Grad. Sch. of Med. and Dent. Sci., TMDU)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-071] Single cell RNA-seq of Neutrophils from Severe COVID-19 Patients

^○Masakazu Ishikawa¹, Hiroshi Ito², YuChen Liu¹, Jumpei Yoshimura², Shuhei Sakakibara¹, Yuji Ogura², Jun Oda², Hisatake Matsumoto², Okuzaki Daisuke¹ (1.IFReC, Osaka Univ., 2.TACCC, Osaka Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-072] Role of calmodulin in sexual reproduction of fission yeast

^○Erina Yamaguchi, Taro Nakamura (Dept. of Biol., Grad. Sch. of Sci., Osaka Met. Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-073] Application of chromosome engineering technology (1): Efficiently micronucleation and chromosome transfer in CHO cells using inhibitors of chromosome distribution mechanisms and mitotic checkpoints

^○Narumi Uno¹, Hiroyuki Satofuka², Hitomaru Miyamoto³, Kazuhisa Honma², Teruhiko Suzuki⁴, Kyotaro Yamazaki³, Ryota Ito¹, Takashi Moriwaki², Kazuma Tomizuka¹, Mitsuo Oshimura², Yasuhiro Kazuki^2,3,5 (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Chromo. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-074] Application of chromosome engineering technology (2):Direct chromosome transfer using human iPS cells as donor cells

^○Hitomaru Miyamoto¹, Narumi Uno², Teruhiko Suzuki³, Kazuma Tomizuka², Yasuhiro Kazuki^1,4 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 3.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 4.CERC, Tottori Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-075] Application of chromosome engineering technology (3) : Development of a novel chromosome transfer technology to use mouse embryonic stem cell as a chromosome donor cell

^○Shiho Sasaki^1,2, Teruhiko Suzuki¹, Mana Yamakawa¹, Hitomaru Miyamoto³, Narumi Uno⁴, Yasuhiro Kazuki⁵, Takahiko Hara^1,2,6 (1.Stem Cell Proj, Tokyo Metropol. Inst. Med. Sci., 2.Grad. Sch., Tokyo Med. Dent. Univ., 3.Div. of Chromo. Biomed. Eng, Grad, Sch. of Med. Sci., Tottori Univ., 4.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 5.CERC, Tottori Univ., 6.Grad. Sch., Tokyo Metropol. Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-076] Application of Chromosome Engineering Technology (4). Elucidation of molecular mechanisms in horizontal propagation of foreign DNA and chromosomes at the Mb scale based on microcells.

^○koya Tajima¹, narumi Uno¹, Ayumu Tonooka¹, Yasuhiro Kazuki^2,3,4, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Chromo. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-077] Application of chromosome engineering technology (5): Construction of top-down HAC derived from chromosome 21 in human iPS cells by megabase deletion using genome editing (CRISPR/Cas9)

^○Masaya Egawa¹, Narumi Uno¹, Hitomaru Miyamoto², Yusuke Ohkame³, Rina Komazaki¹, Yuki Ishizu¹, Yusaku Okano¹, Taku Yunomae¹, Yasuhiro Kazuki^2,3,4, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci, 2.Div. of Chromo. Biomed Eng., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan