[1P-270] Novel Western Blotting Method Adopted For Applying Agarose Native Gel Electrophoresis

^○Chiaki Sakuma¹, Masataka Nakagawa¹, Yui Tokioka¹, Toshiaki Maruyama², Kevin Entzminger², Jonathan K Fleming², Shigeru Okumura², Ryo Sato¹, Takashi Shibata¹, Yasunori Kurosawa¹, Tsutomu Arakawa³, Teruo Akuta¹ (1.Kyokuto Pharm. Ind. Co., Ltd, 2.Abwiz Bio Inc., 3.Alliance Protein Laboratories)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-271] Analysis of protein denaturation and aggregation by agarose native gel electrophoresis

^○Yui Tomioka¹, Chiaki Sakuma¹, Masataka Nakagawa¹, Yasunori Kurosawaa¹, Satoru Nagatoishi², Kouhei Tsumoto^2,3, Matsujiro Ishibashi⁴, Tsutomu Arakawa⁵, Teruo Akuta¹ (1.Kyokuto Pharm. Ind. Co., Ltd, 2.Inst. of Med. Sci., Univ. of Tokyo, 3.Sch. of Eng., Univ. of Tokyo, 4.Fac. of Agric., Kagoshima Univ., 5.Alliance Protein Laboratories)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-272] Cysteine palmitoylation regulates enzymatic activity of lysine deacetylase SIRT1

^○Kota Noritsugu¹, Minami Tamada¹, Takehiro Suzuki², Naoshi Dohmae², Akihiro Ito¹ (1.Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 2.Biomolec. Character. Unit, RIKEN CSRS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-273] Single molecule observation of structural dynamics in the prion-like structural replication reaction of amyloid fibrils

^○Takahiro Nakayama¹, Ono Kenjiro² (1.WPI-NanoLSI, Kanazawa Univ., 2.Neurology, Grad. Sch. of Med., Kanazawa Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-274] Establishment of a mouse model for pseudohyperaldosteronism and investigation of the involvement of Mac-2BP protein in the disease

^○Ayaka Eguchi^1,2, Satoko Arai^2,3, Toru Miyazaki² (1.Fac. of Med., Univ. of Tokyo, 2.IAM, 3.Lab. of Mol. Biomed. for Pathog., Grad. Sch. of Med., Univ. of Tokyo)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-341] Centromere-kinetochore structures revealed by 12x modified expansion microscopy

^○Yasuhiro Hirano¹, Aussie Suzuki², Yasushi Hiraoka¹, Tatsuo Fukagawa¹ (1.Graduate School of Frontier Biosciences, Osaka University, 2.McArdle Laboratory for Cancer Research, University of Wisconsin-Madison)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-342] Roles of the lipid-binding region of an inner nuclear membrane protein Bqt4 in fission yeast

^○Yasuhiro Hirano¹, Tsukino Sato², Ayane Miura³, Kazuya Kabayama^3,2, Koichi Fukase^2,3, Tatsuo Fukagawa¹, Tokuko Haraguchi¹, Yasushi Hiraoka¹ (1.Graduate School of Frontier Biosciences, Osaka University, 2.School of Science, Osaka University, 3.Graduate School of Science, Osaka University)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-343] The ceramide synthase homolog, Tlc4, maintains the nuclear membrane integrity via its translocation from the endoplasmic reticulum to Golgi in fission yeast

^○Yasuhiro Hirano¹, Yusuke Ohno², Akio Kihara², Tatsuo Fukagawa¹, Tokuko Haraguchi¹, Yasushi Hiraoka¹ (1.Graduate School of Frontier Biosciences, Osaka University, 2.Faculty of Pharmaceutical Sciences, Hokkaido University)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-344] Separase regulation mediated by its autocleavage

^○Norihisa Shindo¹, Toru Hirota² (1.Div. Mol. & Cell. Oncol., Miyagi Cancer Ctr. Res. Inst, 2.Div. Exp. Path., Cancer Inst, JFCR)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1P-345] iPSC reprogramming-mediated aneuploidy rescue in the major autosomal trisomy syndromes

^○Akutsu Silvia Natsuko¹, Miyamoto Tatsuo², Oba Daiju³, Tomioka Keita^1,4, Ochiai Hiroshi⁵, Ohashi Hirofumi³, Matsuura Shinya¹ (1.Department of Genetics and Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan, 2.Department of Molecular and Cellular Physiology, Graduate School of Medicine, Yamaguchi University, Ube 755-8505, Japan, 3.Department of Genetics, Saitama Children’s Medical Center, Saitama 330-8777, Japan, 4.Department of Pediatrics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan, 5.Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-0046, Japan)

The 45th Annual Meeting of the Molecular Biology Society of Japan