Best Presentation Award 1

[IYS-04] Microbial network and fermentation characteristics of woody silage prepared with exogenous carbohydrate additives

〇Zhumei Du^1,2, Fuyu Yang¹, Yimin Cai² (1.College of Grassland Science and Technology, China Agricultural University, 2.Japan International Research Center for Agricultural Science (JIRCAS))

In order to effectively use woody feed resources, Pacific Biosciences (PacBio) single-molecule, real-time (SMRT) sequencing was applied to study the microbial network and fermentation characteristics of paper mulberry (PM) silage prepared with exogenous additives such as corn meal (CM) and rice bran (RB). PM is rich in nutrients and contains more than 26% crude protein on dry matter basis. After ensiling, the microbial alpha diversity and relative abundance of PM, CM and RB decrease due to the anaerobic environment and acidic condition. CM-treated PM silage can accelerate the conversion of the dominant microbial community from harmful bacteria to lactic acid bacteria, and promote lactic acid fermentation. When RB is used to treat PM silage, Enterobacter and Clostridium species quickly become the main bacterial community during ensiling, leading to butyric acid fermentation and protein decomposition. Compared with RB, CM can increase fermentation substrates, change the microbial community structure, affect metabolic pathways, and improve the flavor and quality of silage. Therefore, PM can be used as a potential high-protein silage in animal production.