[1P-104] Modification of Fab as the fiducial mark for cryo-EM analysis using the epitope tag-grafting technique.

^〇Yukari Tanaka¹, Yuriko Adachi¹, Mika Hirose², Takayuki Kato², Mika K. Kaneko³, Yukinari Kato³, Terukazu Nogi¹ (1.Graduate School of Medical Life Science, Yokohama City University, 2.Institute for Protein Research, Osaka University, 3.Tohoku University Graduate School of Medicine)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-105] Preliminary structural analysis of Clostridium perfringens CppB-CppA covalent complex at the host-cell adhesion site of pili by cryo-electron microscopy and X-ray crystallography

^〇Yasuhiro Nonaka¹, Eiji Tamai², Hiroshi Sekiya², Takanori Nakamura¹, Shigehiro Kamitori^1,3 (1.Faculty of Medicine, Kagawa University, 2.College of Pharmaceutical Science, Matsuyama University, 3.Research Facility Center for Science & Technology, Kagawa University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-106] Structural analysis of the non-Hanks-type protein kinase TpkB provides new insights into the function of PrkA/YeaG family

^〇Masayuki Torii¹, Kazutoshi Tani², Ryo Kanno³, Malgorzata Hall⁴, Akira Mizoguchi², Bruno M. Humbel⁴, Ryoji Masui¹ (1.Grad. Sch. Sci., Osaka Metropolitan Univ.,, 2.Grad. Sch. Med., Mie Univ., 3.Quantum Wave Microscopy Unit, OIST, 4.Imaging Sect., OIST)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-107] Functional analysis of Ccdc9 and Ccdc9b genes in mouse spermatogenesis

^〇Kao Nakajima¹, Yuta Nihongaki², Yukiko U. Inoue³, Kazuma Waguri¹, Takayoshi Inoue³, Takanari Inoue², Kyosuke Shinohara¹ (1.Tokyo University of Agriculture and Technology, 2.Johns Hopkins University, 3.National Center of Neurology and Psychiatry)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-108] Structural study to develop nuclear transportation inhibitor targeting of ependymoma-driving ZFTA-RELA fusion Protein

^〇Shoma Imamura¹, Ruri Kojima², Yoichi Yamazaki¹, Kento Yonezawa¹, Daisuke Kawauchi³, Shinya Oohata⁴, Hironari Kamikubo ¹, Sachiko Toma ¹ (1.Nara Institute of Science and Technology (NAIST), Graduate School of Advanced Science and Technology, 2.Musashino University, Faculty of Pharmacy, 3.National Center of Neurology and Psychiatry (NCNP), 4.Musashino University, Faculty of Pharmacy, Research Institute of Pharmaceutical Sciences)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-109] X-ray crystallographic structure analysis of L-methionine gamma-lyase 2 in complex with L-methionine from Glycine max

^〇Saki Hashimoto¹, Haruna Makino¹, Kanta Inoue¹, Tomoaki Inoue¹, Daiki Yokoyama², Atsushi Okawa², Takuya Teshima³, Kenji Matsui³, Kenji Inagaki², Tomoo Shiba¹ (1.Dept. Appl. Biol., Grad. Sch. Sci. and Tech., Kyoto Inst. of Tech., 2.Grad. Sch. Env. & Life Sci., Okayama Univ., 3.Grad. Sch. Sci. & Tech., Yamaguchi Univ.)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-110] Structural study of protein arginine methyltransferase 1 in complex with FET family proteins

^〇Nobuyuki E. Endo¹, Hinako K. Koshimura¹, Akihiro K. Kawamoto², Mika H. Hirose², Akiko I. Ikegami¹, Miwa K. Kuzuhara¹, Hiroaki D. Daitoku^3,4, Koichiro K. Kako⁵, Yoichi Y. Yamazaki¹, Kento Y. Yonezawa¹, Takayuki K. Kato², Akiyoshi F. Fukamizu^3,4, Hironari K. Kamikubo¹, Sachiko T-F. Toma-Fukai^1,4 (1.Graduate School of Science and Technology, Nara Institute of Science and Technology, 2.Institute for Protein Research, Osaka University, 3.Tsukuba Advanced Research Alliance, University of Tsukuba, 4.Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST), 5.Faculty of Life and Environmental Sciences, University of Tsukuba)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-111(1T05a-09)] Functional and Structural Studies of a Novel Cas9 from Hot Springs

^〇Osamu Kikko¹, Shunsuke Matsumoto², Sonoko Ishino², Hiroyuki Matsumoto³, Masahiro Noguchi³, Tomoyuki Numata², Yoshizumi Ishino² (1.Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 2.Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 3.Takara Bio Inc.)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-112] Structure and function of PET-degrading enzyme Cut190 and its comparison with related enzyme LCC

^〇Riri Takenaka¹, Fumiya Kondo², Masayuki Oda^1,2 (1.Faculty of Life and Environmental Sciences, Kyoto Prefectural University, 2.Graduate School of Life and Environmental Sciences, Kyoto Prefectural University)

The 96th Annual Meeting of the Japanese Biochemical Society

[1P-113] Cryo-EM structure of protein arginine methyltransferase 1 in complex with cofactor SAH

^〇Sachiko Toma-Fukai^1,2, Akihiro Kawamoto³, Mika Hirose³, Miwa Kuzuhara¹, Akiko Ikegami¹, Hiroaki Daitoku⁴, Koichiro Kako⁵, Jun-Dal Kim^2,6, Susumu Uchiyama⁷, Takayuki Kato³, Toshiyuki Shimizu⁸, Hironari Kamikubo^1,8, Akiyoshi Fukamizu^2,4 (1.Graduate School of Science and Technology, Nara Institute of Science and Technology, 2.Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST), 3.Institute for Protein Research, Osaka University, 4.Tsukuba Advanced Research Alliance, University of Tsukuba, 5.Faculty of Life and Environmental Sciences, University of Tsukuba, 6.Institute of Natural Medicine, University of Toyama, 7.Graduate School of Engineering Division of Science and Biotechnology, Osaka University, 8.Graduate School of Pharmaceutical Sciences, The University of Tokyo)

The 96th Annual Meeting of the Japanese Biochemical Society