[1P-1331] Generation of genome editing mice using cryopreserved embryos and electroporation.

〇Yayoi Kunihiro¹, Yuko Kotani¹, Yuko Yamauchi¹, Yoshihiro Uno¹, Kazuto Yoshimi^1,2, Tomoji Mashimo^1,2 (1.Inst. of Exp.Anim. Sci., Grad. Sch. of Med., Univ. of Osaka, 2.Genome editing Center, Grad. Sch. of Med., Univ. of Osaka)

ConBio2017

[1P-1332] Correction of R778L mutation in Wilson's disease iPS cells using CRISPR/Cas9

〇Gou Takahashi¹, Tomoko Kato¹, Szu Yin Hsu¹, Yuichiro Miyaoka¹ (1.Tokyo Metropolitan Institute of Medical Science, Regenerative Medicine Project)

ConBio2017

[1P-1333] Gene editing via NHEJ/MMEJ is an easy, quick and efficient way to establish gene modified human ES cell lines

〇Norihiko Kobayashi¹, Akira Yuo¹, Kumiko Saeki¹ (1.Dept. of Dz. Cont., RI., NCGM)

ConBio2017

[1P-1334] Evaluation of recombinant enzyme-free seamless DNA cloning methods.

〇Ken Motohashi¹ (1.Dept. of Biores. Environ. Sci., Kyoto Sangyo Univ.)

ConBio2017

[1P-1335] Development of a guide RNA for inducing ADAR-mediated A-to-I RNA editing into the target-site

〇Kanako Nose¹, Ryoma Noguchi¹, Rina Hoshino¹, Naoki Masuda¹, Hiroyuki Nakagawa², Masatora Fukuda¹ (1.Dept. Chem., Fac. Sci., Fukuoka Univ., 2.Dept. Div. Biol., Fac. Sci., Fukuoka Univ.)

ConBio2017

[1P-1336] Simple and sensitive electrophoretic detection of covalently closed DNA circles formed by in vitro ligation

〇Akiko Mizutani^1,2, Hidetoshi Inoko², Masafumi Tanaka² (1.Faculty of Medical Health, Teikyo Heisei Univ., 2.Dep. of Mol. Life Sci. Tokai Univ. Sch. of Med.)

ConBio2017

[1P-1337] Consideration of conditions for the generation of genome edited mice by electroporation with high efficiency and high survival rate

〇Michihiko Sugimoto¹, Daisuke Ariyasu¹, Kei-ichiro Ishiguro², Shingo Ito³, Kimi Araki¹ (1.Dev. Genet., IRDA, Kumamoto Univ., 2.Chromosome Biol., IMEG, Kumamoto Univ., 3.Pharm. Microbiol., Grad. Sch. of Life Sci., Kumamoto Univ.)

ConBio2017

[1P-1338] Preparation of RNase H2 gene-deficient cell line of NIH3T3 mouse fibroblasts

〇Motoki Tsukiashi¹, Kenji Kojima¹, Misato Baba¹, Teisuke Takita¹, Kiyoshi Yasukawa¹ (1.Div. of Food Sci. and Biotechnol., Grad. Sch. Agric., Kyoto Univ.)

ConBio2017

[1P-1339] TALEN-mediated knock-in at the intron region of the Sericin 1 gene of silkworms, Bombyx mori, for a recombinant protein production.

〇Ken-ichiro Tatematsu¹, Mai Hayakawa¹, Keiro Uchino¹, Hideki Sezutsu¹ (1.Inst. of Agrobiol. Sci., Transgen. Silkworm Res. Unit)

ConBio2017

[1P-1340] Development of global DNA methylation level detection system using MBD- and CXXC-luciferase

〇Yuji Baba¹, Isao Karube¹, Wataru Yoshida¹ (1.Graduate School of Bionics, Tokyo University of Technology)

ConBio2017