[2Cp] 食品分析

[2Cp-05] Development of Amine Derivatization LC-MS/MS Technique for Non-Targeted De Novo Peptide Sequencing in Rat Plasma After Soy Protein Administration

肖 懿芝¹、樫原 里咲¹、松井 利郎²、*田中 充² (1. 九大・院・生資環、2. 九大・院・農)

キーワード： N-スクシニミジル-7-メトキシクマリン-3-カルボキシレート（クマリン）、ペプチドミクス、LC-MS/MS、アミン誘導体化、デノボシーケンシング

Purpose: This study aimed to develop a novel peptide identification method using amine derivatization techniques for de novo sequencing of peptides, addressing challenges in non-targeted peptides analysis in rat plasma after soy protein isolate (SPI) administration.Methods: Peptides were derivatized with various amine derivatization reagents including N-succinimidyl-7-methoxycoumarin-3-carboxylate (Cou) and then applied to LC-qTOF/MS for comparison.Results: Compared to other derivatization methods, Cou derivatization-aided LC-MS/MS method found that peptide bond cleavage occurred sequentially from the N-terminus of the coumarin tag. The present method achieved peptide sequencing up to 10 residues and identified a variety of oligopeptides from plasma of male SD rats after ingestion of SPI.