[3P-0590] The novel electroporation of a Water-in-oil Droplet was applied to cytomorphology modification

〇Rika Numano¹, Minako Matsuo¹, Naobumi Kimura¹, Hirofumi Kurita¹, kohjiro Matsumoto², Kazuki Nakao³ (1.Department of Applied Chemistry and Life, Toyohashi University of Technology, 2.Nepa Gene Co., Ltd., 3.Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo,)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0591] Development of low cytotoxic electroporation system

〇Ryuto Shinozaki¹, Minako Matsuo¹, Kohjiro Matsumoto², Hirohumi Kurita¹, Rika Numano¹ (1.Toyohashi University of Technology, Development of Applied Chemistry and Life Science, 2.Nepa Gene Co., Ltd.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0592] Gene editing without artificial nucleases : Comparison between single-stranded DNA and Tailed Duplex

〇Kento Sato¹, Tetsuya Suzuki¹, Hidehiko Kawai¹, Hiroyuki Kamiya¹ (1.Grad. Sch. of Biomed. and Health Sci.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0593] Gene editing without artificial nucleases:Establishment of a high-throughput assay system using fluorescent proteins

〇Kentarou Yazama¹, Tetsuya Suzuki^1,2, Hidehiko Kawai^1,2, Hiroyuki Kamiya^1,2 (1.Sch. of Pharma. Sci., Univ. of Hiroshima, 2.Grad. Sch. of Biomed. and Health Sci.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0594(4W-06-7)] Illegitimate translation and human diseases: a new tool to study the molecular mechanism of translation

〇Yoichi Gondo^1,2, Shigeru Makino² (1.Molec. Life Sci., Tokai Univ. Sch. Med., 2.RIKEN BioResource Res. Ctr.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0595] Allele-selective genome editing by fine-tuning CRISPR-Cas9 activity

〇Masaki Kawamata¹, Ryota Kimura¹, Atsushi Suzuki¹ (1.Div. of Org. Reg., Med. Inst. of Bioreg., Kyushu Univ.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0596] Smart in-frame donor cassette knock-in system via Crispr-Cas9 mediated gene targeting

〇Riki Ishibashi^1,2, Nanami Ido^1,2, Fumiko Toyoshima^1,2 (1.Dept. of Bios. Sci., Lab. of Tissue Homeostasis, Inst. for Front. and Med. Sci., Kyoto Univ., 2.Dept. of Mammal. Regu. Net., Lab. of Cell Divi. and Diff., Grad. Sch. of Biost. Kyoto Univ.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0597] Practice and technical barrier of intra-embryonic large cassette knock-in by Adeno-Associated Viral vector with Cas9 ribonucleoprotein electroporation.

〇Naoaki Mizuno¹, Eiji Mizutani¹, Hideyuki Sato¹, Mariko Kasai¹, Tomoyuki Yamaguchi¹, Hiromitsu Nakauchi^1,2 (1.Div. of SCT, IMSUT, Univ. of Tokyo, 2.Dept. of Genetics, Stanford Univ. School of Medicine.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0598(3AW-05-8)] Generation of imprinting disorder model mice by epigenome editing

〇Takuro Horii¹, Sumiyo Morita¹, Shinjiro Hino², Mika Kimura¹, Yuko Hino², Hiroshi Kogo³, Mitsuyoshi Nakao², Izuho Hatada¹ (1.Biosignal Genome Resource Center, IMCR, Gunma Univ., 2.Inst. of Mol. Embryo. and Genet., Kumamoto Univ., 3.Grad. Sch. of Med., Gunma Univ.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan

[3P-0599] Application of butterfly cytotoxin to generate the transgenic silkworms, which produce sericin cocoons for the utilization of cell culture.

〇Chihiro Umehara¹, Keiko Takaki¹, Hajime Mori¹, Eiji Kotani¹ (1.Dept.of Applied Biol.,Kyoto Inst.Tech.)

The 42nd Annual Meeting of the Molecular Biology Society of Japan