[2P-220] Development of an ER-coupled in vitro translation system reconstituted with human factors

^○Mayumi Sunagawa, Tsubasa Inoue, Mizuki Okabayashi, Fuka Kanemitsu, Saaya Toyota, Kodai Machida, Hiroaki Imataka (Dept. of Appl. Chem., Grad. Sch. of Eng., Univ. of Hyogo)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-221] Effect of N-terminal amino acid sequence on the protein synthesis using a reconstituted cell-free protein synthesis system (PUREfrex®)

^○Takashi Kanamori, Tomoe FuseMurakami (GeneFrontier Corp.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-222] Identification of modification enzymes responsible for two pseudouridines of the anti-Shine-Dalgarno sequence region in Thermus thermophilus 16S rRNA

^○Mone Nobeoka¹, Yusuke Kuwana¹, Kohsuke Kamizaki¹, Kyosuke Fukuda¹, Shizuka Arakawa¹, Takashi Yokogawa², Chie Takemoto³, Ryota Yamagami¹, Hiroyuki Hori¹ (1.Grad. Sch. of Sci., Univ. of Ehime, 2.Dept. of Eng., Univ. of Gifu, 3.RIKEN BDR)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-223] Elucidation of relationship between thermo-tolerance and cell death via translational suppression and initiator tRNA degradation

^○Yuiko Aoki¹, Yusuke Umemoto², Suzuka Nagahusa³, Akihisa Takahashi⁴, Kenichi Ijiri⁵, Takashi Ohtsuki^1,2,3, Kazunori Watanabe^1,2,3,5 (1.Grad. Sch. Of Interdis. Sci. and Eng. in Health Systems, Okayama Univ., 2.Grad. Sch. of Nat. Sci. and Tech, Okayama Univ., 3.Sch. of Eng, Okayama Univ., 4.Gunma Univ. Heavy Ion Med. Center, 5.Radioisotope Center, Tokyo Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-224] In vitro reconstruction of the translation factors regeneration system for self-replicating artificial cells

^○Katsumi Hagino¹, Keiko Masuda⁴, Yoshihiro Shimizu⁴, Norikazu Ichihashi^1,2,3 (1.Department of Life Science, Graduate School of Arts and Science, The University of Tokyo, 2.Komaba Institute for Science, The University of Tokyo, 3.Research Center for Complex Systems Biology, Universal Biology Institute, The University of Tokyo, 4.Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-225] Elucidation of the effects of polyamines on CAG repeat-derived translation.

^○Kodai Machida, Kanta Kitajiri, Hiroaki Imataka (Dept. of Appl. Chem., Grad. Sch. of Eng., Univ. of Hyogo)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-226] EEF1A1 and EEF1A2 complement each other in regulating protein synthesis and cell cycle in breast cancer cells.

^○Mohamad Jamous, Masaki Hosogane, Keiko Nakayama (Department of Cell Proliferation, ART, Tohoku University Graduate School of Medicine, Sendai, Japan)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-227] Seeking for a practical method for preparation of wheat threonyl-tRNA synthetase

^○Takumi Nishioka, Haruyuki Furukawa, Chie Tomikawa, Kazuyuki Takai (Dept. of Materials Sci. and Biotech., Grad. Sch. of Sci. and Eng., Ehime Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-228] IbpA, an Escherichia coli small Hsp, is an aggregation-sensor that regulates its own translation and heat shock response at a translational level.

^○Tsukumi Miwa, Hideki Taguchi (Cell Biology Center, IIR, Tokyo Tech)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[2P-229] The mechanism of cap-independent translation under hypoxic stress condition

^○Kaiki Noguchi¹, Kosuke Taniguchi¹, Mari Takahashi², Takumi Tomohiro¹, Akira Fukao¹, Hirotatsu Imai³, Akio Yamashita³, Takuhiro Ito², Toshinobu Fujiwara¹ (1.Dept. of Pharmacy, Kindai Univ., 2.Center for Biosystems Dynamics Research RIKEN, 3.Dept. of Investigative Medicine, Univ. of the Ryukyus)

The 46th Annual Meeting of the Molecular Biology Society of Japan