[3P-0627] Annexin A5 is essential for the maintenance of function of tooth and jaw bones through biting force.

^○Hisashi Ideno¹, Ayako Mochizuki², Koichiro Komatsu¹, Kazuhisa Nakashima¹, Toshihiko Amemiya³, Yasuo Imanishi⁴, Yoshinori Arai³, Yoichi Ezura⁵, Tomio Inoue², Akira Nifuji¹ (1.Dept Pharmacol., Tsurumi Univ Sch Dent Med., 2.Dept Oral Physiol., Showa Univ Sch Dent., 3.Dept Oral Maxillofac Radiol., Nihon Univ Sch Dent., 4.Dept Metab Endocrinol., Osaka City Univ Sch Med., 5.Fac Med Tech, Teikyo Univ)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0628] In vivo monitoring of gRNA expression based on translational nonsense codon suppression via suppressor tRNA in plants

^○Kazuhito Akama¹, Tatsuki Uda¹, Yasushi Yukawa² (1.Fac. Life & Environ. Sci., Shimane Univ., 2.Gradu. School Natural Sciences., Nagoya City Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0629] Contribution of ROS and Importance of Endocytosis in Surface Discharge Gene Transfer Method

^○Tomoki Oda¹, Tatsuya Hiramatsu¹, Hiroko Hirashige¹, Yoshihisa Ikeda¹, Yugo Kido^{1, 2}, Susumu Satoh^{1, 3}, Masafumi Jinno^{1, 3} (1.Ehime Univ., 2.Pearl Kogyo Co. Ltd., 3.i-Gene Corp.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0630] Single-cell level analysis of genome editing outcomes induced by CRISPR-Cas9

^○Daiki Kondo^{1, 2}, Gou Takahashi¹, Yuji Morishita³, Minato Maeda^{1, 4}, Yuichiro Miyaoka^{1, 2} (1.Reg. Med. Project, Tokyo Met. Inst. of Med. Sci., 2.Grad. Sch. of Tokyo Med. and Dent. Univ., 3.On-chip Biotechnologies, Inc., 4.Dept. of Biosci., Sch. of Sci. and Tech., Chuo Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0631] The editing and screening of silkworm BAC for construction of silkworm artificial chromosomes

^○Yoshino Tonooka¹, Masato Hino², Toshiaki Fujimoto³, Hiroaki Mon², Jaeman Lee², Ryosuke Fujita², Yuuji Yasukouchi⁴, Ken Sahara³, Takahiro Kusakabe² (1.Grad. Sch. of Biores. Bioenv. Sci., Univ. of Kyushu, 2.Fac. Agr., Univ. of Kyushu, 3.Fac. Agr., Univ. of Iwate, 4.Nat. Agri. & Food Res. Org. (NARO))

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0632] Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

^○Kanae Aoki, Keita Okada, Kota Sugio, Teruyuki Tabei, Katsunori Imai, Yuki Bonkohara, Yusuke Kamachi (Sch. of Env. Sci. and Eng., Kochi Univ. of Tech.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0633] Effects of abnormal fusion transcript on phenotype in knockout mouse.

^○Miyuki Shindo¹, Kohji Okamura² (1.Div. of Lab. Anim. Resour., NCCHD, 2.Dept. of Sys. BioMed., NCCHD)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0634] Targeted single nucleotide substitutions in Arabidopsis thaliana nuclear genes using cytidine deaminase from Burkholderia cenocepacia

^○Ayako Hosoda, Nobuhiro Tsutsumi, Shinichi Arimura (Grad. Sch. of Agr. and Life Sci., Univ. of Tokyo)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0635] Precise gene editing in iPS cells via Prime editing and FACS enrichment

^○Ryo Niwa^{1, 2}, Alexander Liu^{1, 3}, Suji Lee^{1, 2}, Tomoko Matsumoto¹, Knut Woltjen¹ (1.CiRA, Kyoto Univ., 2.Grad. Sch. of Med., Kyoto Univ., 3.Dept. of Sci., Kyoto Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0636] A dark-leakless photoactivatable Cre-loxP recombination system improved by a chemogenetic degradation tag

^○Fuun Kawano, Moritoshi Sato (Graduate school of Arts and Sciences, UTokyo)

The 44th Annual Meeting of the Molecular Biology Society of Japan