[3P-0669] Application of chromosome engineering technology (8): Generation of internalizing monoclonal antibodies by fully human antibody-producing mice and their comprehensive analyses by cytotoxicity-based assay systems

^○Yayan Wang^{1, 2}, Hiroyuki Satofuka³, Kyotaro Yamazaki¹, Shusei Hamamichi^{3, 4}, Takeshi Fukuhara⁵, Rafique Abdur⁶, Nana Osako⁶, Iori Kanazawa⁷, Takeshi Endo⁷, Naomi Miyake⁸, Kazuhisa Honma⁷, Yuichi Nagashima⁸, Satoshi Abe⁷, Takashi Moriwaki^{1, 3, 8}, Xu Gao², Mitsuo Oshimura⁷, Yuji Ito⁶, Yasuhiro Kazuki^{1, 3, 8} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Dep. of Bio. Chem. and Mol. Bio., Harbin Med. Univ., 3.CERC, Tottori Univ., 4.Res. Inst. for Dis. of Old Age, Sch. of Med., Juntendo Univ., 5.Dep. of Neu., Grad. Sch. of Med., Juntendo Univ., 6.Dep. of Chem. and Biosci., Grad. Sch. of Sci. and Eng., Kagoshima Univ., 7.Trans Chromosomics Inc., 8.Div. of Mol. Genet. and Biofunc., Grad. Sch. of Med. Sci., Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0670] Application of chromosome engineering technology (9): Generation of internalizing monoclonal antibodies using fully human antibody-producing mice and their comprehensive analyses by cytotoxicity-based assay systems

^○Kazuto Shimoya^{1, 2}, Hiroyuki Satofuka², Kazuki Sadakane^{1, 2}, Genki Hichiwa^{1, 2}, Yasuhiro Kazuki^{1, 2, 3} (1.Dev. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Sch. of Life Sci., Facul. of Med., Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0671] Application of chromosome engineering technology (10): Development of a simple technique for producing monoclonal antibodies using protein probes

^○Kazuki Sadakane^{1, 2}, Hiroyuki Satofuka², Hiroshi Tanaka³, Maria Tanaka³, Kayoko Morimoto³, Kazuto Shimoya^{1, 2}, Genki Hichiwa^{1, 2}, Yasuyuki Kurihara⁴, Mitsuo Oshimura³, Yasuhiro Kazuki^{1, 2} (1.Div. of Mol. Genet. and Biofunc., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Trans Chromosomics Inc., 4.Lab. of Mol. Bio., Fac. of Eng. Sci., Yokohama Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0672] Application of chromosome engineering technology (11): Development of therapeutic antibody using fully human antibody-producing animals combined with phage display system

^○Genki Hichiwa^{1, 2}, Hiroyuki Satofuka², Narumi Uno³, Rafique Abdur⁴, Kazuma Tomizuka³, Yuji Ito⁴, Yasuhiro Kazuki^{1, 2} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 4.Dep. of Chem. and Biosci., Grad. Sch. of Sci. and Eng., Kagoshima Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0673] Application of chromosome engineering technology (12): Development of mice producing human antibodies with expanded diversity by replacing human IgHD sequences with chemically synthesized IgHD sequences

^○Yuko Wakasa¹, Takashi Moriwaki^{2, 3}, Satoshi Abe⁴, Yuko Yajima¹, Narumi Uno¹, Yasuhiro Kazuki^{2, 3}, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Trans Chromosomics Inc.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0674] Application of chromosome engineering technology (13): Development of inducibly diversified antibody-expressing cell library that enables antibody phenotypic screening

^○Nanami Hashimoto¹, Wataru Sasagawa¹, Narumi Uno¹, Hiroyuki Satofuka², Yasuhiro Kazuki^{2, 3}, Akitsu Hotta⁴, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 4.CiRA, Kyoto Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0675] Application of chromosome engineering technology (14): Development of a diversified induced expression cell library for phenotypic screening of functional peptides

^○Hiromu Maruko¹, Yuki Okuyama¹, Nanami Hashimoto¹, Narumi Uno¹, Yuki Ishizu¹, Yasuhiro Kazuki^{2, 3}, Akitsu Hotta⁴, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.CiRA, Kyoto Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0676] Application of chromosome engineering technology (15): Construction of an exon skip evaluation system in C2C12 cells carrying the full length of the human DMD gene

^○Yukiko Takino¹, Narumi Uno¹, Yasuhiro Kazuki^{2, 3}, Yuji Ito⁴, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Dep. of Chem. and Biosci., Grad. Sch. of Sci. and Eng., Kagoshima Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0677] Application of chromosome engineering technology (16): Design of highly functionalized mesenchymal stem cell medicine using human artificial chromosomes(HAC)

^○Chihiro Onodera¹, Natsumi Miyazaki¹, Narumi Uno¹, Rena Yamauchi^{1, 2}, Yuki Ishizu¹, Yuko Wakasa¹, Ryota Mayuzumi³, Hiroyuki Satofuka⁴, Yasuhiro Kazuki^{3, 4}, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Technopro, Inc., Tokyo Branch, 3.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 4.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0678] Application of chromosome engineering technology (17): Investigating a novel mammalian promoter activation sequence derived from 5ʹ-upstream sequence of housekeeping gene

^○Natsumi Miyazaki¹, Chihiro Onodera¹, Ryota Mayuzumi², Narumi Uno¹, Yuki Ishizu¹, Wakana Takeuchi¹, Chiaki Hando¹, Hitomaru Miyamoto², Yusuke Ohkame³, Shuta Takata³, Yasuhiro Kazuki^{2, 3}, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan