[3P-0679] Application of chromosome engineering technology (18): Construction of Top-down Human Artificial Chromosome in Human iPS cells by Megabase-scale Chromosomal Truncations using CRISPR/Cas9

^○Masaya Egawa¹, Narumi Uno¹, Hitomaru Miyamoto², Yusuke Ohkame³, Rina Komazaki¹, Yuki Ishizu¹, Taku Yunomae¹, Yasuhiro Kazuki^{2, 3}, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci, 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0680] Application of chromosome engineering technology (19): Development of new chromosome transfer technology for cell therapy using human iPS cells as a chromosome donor.

^○Hitomaru Miyamoto¹, Narumi Uno², Ryota Mayuzumi¹, Motoki Kawasaki¹, Hiroyuki Satofuka³, Kazuma Tomizuka², Yasuhiro Kazuki^{1, 3} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 3.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0681] Application of chromosome engineering technology (20): Construction of human iPS cells with partial monosomy of chromosome 21 and search for essential regions

^○Rina Komazaki¹, Yuki Ishizu¹, Masaya Egawa¹, Taku Yunomae¹, Yusuke Ohkame², Shuta Takata², Chihiro Yoshimatsu³, Kyoutarou Yamazaki³, Teruhiko Suzuki⁴, Yasunori Aizawa⁵, Narumi Uno¹, Yasuhiro Kazuki^{2, 3}, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.Sch. Life Sci. Tech., Tokyo Tech.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0682] Application of chromosome engineering technology (21) : Development of quantitative screening system for essential genomic elements and its application to human iPS cells.

^○Hikaru Kurasawa^{1, 2}, Yuki Ishizu³, Rina Komazaki³, Narumi Uno³, Kazuma Tomizuka³, Teruhiko Suzuki⁴, Yusuke Ohkame⁵, Shuta Takata⁵, Yasuhiro Kazuki⁵, Yasunori Aizawa^{1, 2} (1.Sch. Life Sci. Tech., Tokyo Tech., 2.KISTEC, 3.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0683] Application of chromosome engineering technology (22) : Development of a method for identification of essential genes

^○Teruhiko Suzuki¹, Mana Yamakawa¹, Anna Suzuki¹, Narumi Uno², Kazuma Tomizuka², Yasunori Aizawa³, Takahiko Hara^{1, 4, 5}, Yasuhiro Kazuki⁶ (1.Stem Cell Proj, Tokyo Metropol. Inst. Med. Sci., 2.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 3.Sch. Life Sci. Tech., Tokyo Tech., 4.Grad. Sch., Tokyo Med. Dent. Univ., 5.Grad. Sch., Tokyo Metropol. Univ., 6.CERC, Tottori Univ)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0684] Degradation of KRAS mutants by a Novel Chemical Knockdown Technology of CANDDY

^○Satoshi Imanishi¹, Lijuan Huang¹, Shoko Itakura¹, Masamichi Ishizaka^{1, 2}, Tomohiro Yamaguchi^{1, 2}, Yoichi Iwasaki^{1, 2}, Etsuko MiyamotoSato^{1, 3, 2} (1.M-S. Mol. Med. Sci. Lab., Tokyo Univ. of Sci., 2.R&D Dep., FuturedMe Inc., 3.Grad Sch. of Biol. Sci., Tokyo Univ. of Sci)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0685] Degradation of Undruggable Target by a Novel Chemical Knockdown Technology of CANDDY

^○Lijuan Huang¹, Satoshi Imanishi¹, Shuichi Mori¹, Kaisei Sai¹, Yoichi Iwasaki^{1, 2}, Etsuko MiyamotoSato^{1, 3, 2} (1.EM-S Mol. Med. Sci. Lab., Res. Inst. for Sci. & Tech., Tokyo Univ. of Sci., 2.R&D Dept., FuturedMe Inc., 3.Grad. Sch. of Biol. Sci., Tokyo Univ. of Sci.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0686] Development of SARS-CoV-2 Main Protease Degraders Based on A Novel Chemical Knockdown Technology \"CANDDY\"

^○Shuichi Mori¹, Kaisei Sai¹, Satoshi Imanishi¹, Lijuan Huang¹, Yoichi Iwasaki^{1, 2}, Etsuko MiyamoaoSato^{1, 3, 2} (1.EM-S. Mol. Med. Sci. Lab., Res. Inst. for Sci. & Tech., Tokyo Univ. of Sci., 2.R&D Dept., FuturedMe Inc., 3.Grad. Sch. of Biol. Sci., Tokyo Univ. of Sci.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0687] Development of the COVID-19 infection prevention strategy by a novel chemical knockdown technology CANDDY

^○Huicheng Qi¹, Shuichi Mori¹, Satoshi Imanishi¹, Lijuan Huang¹, Yoichi Iwasaki^{1, 2}, Etsuko MiyamotoSato^{1, 3, 2} (1.EM-S Mol. Med. Sci. Lab., Res. Inst. for Sci. & Tech., Tokyo Univ. of Sci., 2.R&D Dept., FuturedMe Inc., 3.Grad. Sch. of Biol. Sci., Tokyo Univ. of Sci.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0688] Development of a novel anticancer substance against T-cell acute lymphocytic leukemia by modifying the skeletal structure of the natural product Rumbrin

^○Tomoya Higashi¹, Yuki Fujita¹, Chihiro Yoshida¹, Chihiro Nakata^{2, 3}, Shota Hoyano^{2, 4}, Fumiya Seki^{2, 5}, Nobuo Kitada^{1, 6}, Takashi Hirano¹, Takahiko Hara^{2, 3, 4}, Shojiro Maki^{1, 6} (1.Grad. Sch. of Info. Sci., Univ. of Electro-Communications, 2.Stem Cell Project, Tokyo Metro. Inst. of Med. Sci., 3.Grad. Sch. of Med. and Dent. Sci., Tokyo Med. and Dent. Univ., 4.Grad. Sch. of Sci., Tokyo Metro. Univ., 5.Fac. of Sci. and Engi., Chuo Univ., 6.Cent. for Neuro. and Biomed. Engi., Univ. of Electro-Communications)

The 44th Annual Meeting of the Molecular Biology Society of Japan