[3P-070] Cofactor reconstitution of radical SAM enzyme that is presumed to build ladder-like hydrophobic group in ladderane lipids.

^○Masaya Mima¹, Hirinori Suemune², Tesshin Uegaki², Tomoya Hino², Ken Takai³, Shingo Nagano² (1.Dept. Chem. Biotech., Grad. Schl. Eng., Tottori Univ., 2.Dept. Chem. Biotech., Grad. Schl. Eng., Tottori Univ., 3.JAMSTEC D-SUGAR)

The 92nd Annual Meeting of JBS

[3P-071] Structural biological research for elucidation of Interferon alpha secretion mechanism

^○Honoka Watanabe¹, Toshiki Wada², Masaki Unno^1,3 (1.Graduate School of Science and Engineering, Ibaraki University, 2.Department of Immunology, Kanazawa Medical University, 3.Frontier Research Center for Applied Atomic Sciences, Ibaraki University)

The 92nd Annual Meeting of JBS

[3P-072] Analysis of intracellular localization and membrane topology of a dually acylated membrane protein, ANKRD22

Takuro Hosokawa, Aoi Otsuka, Natsuko Iwamoto, Mayu Shichita, Misato Nishiue, Koko Moriya, ^○Toshihiko Utsumi (Graduate School of Sciences and Technology for Innovation, Yamaguchi University)

The 92nd Annual Meeting of JBS

[3P-073] Sequential ubiquitination of ribosomal protein uS3 triggers the degradation of non-functional 18S rRNA

^○Takato Sugiyama, Sihan Li, Misaki Kato, Ken Ikeuchi, Atsushi Ichimura, Yoshitaka Matsuo, Toshifumi Inada (Graduate School of Pharmaceutical Sciences, Tohoku University)

The 92nd Annual Meeting of JBS

[3P-074] Identification of a ubiquitin ligase which stabilizes Smad6 and inhibits BMP signaling

^○Takanori Hironaka¹, Michio Nakaya^1,2, Yuki Ohba¹, Akiomi Nagasaka¹, Hidetaka Kosako³, Hitoshi Kurose¹ (1.Dept. of Pharmacol. and Toxicol. Kyushu, 2.AMED-PRIME, 3.Institute of Advanced Medical Sciences, Tokushima University)

The 92nd Annual Meeting of JBS

[3P-075] Functional analysis of ubiquitin ligase RNF183 up-regulated under hypertonic conditions

^○Takumi Okamoto, Yan Wu, Kazunori Imaizumi, Masayuki Kaneko (Department of Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University)

The 92nd Annual Meeting of JBS

[3P-076] Study on functions of dual enzymatic activity of PRMT8 using inactive mice

^○Saori Tabara¹, Jun-Dal Kim², Haruka Mori³, Weizhe Lu⁴, Akiyoshi Fukamizu² (1.Graduate School of Life and Environmental Sciences, University of Tsukuba, 2.Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 3.Faculty of Life and Environmental Sciences, University of Tsukuba, 4.Ph.D. Program in Human Biology, School of Integrative Global Majors (SIGMA), University of Tsukuba)

The 92nd Annual Meeting of JBS

[3P-077] Poly-ADP-ribosylation reaction by PARP-1: Importance of cooperative intermolecular interaction during DNA repair by WGR domain

^○Shigeru Negi, Mizuki Okunaga, Midori Tsuruta, Masashi Mashimo (Doshisha Women's University Faculty ofPharmaceutical Science)

The 92nd Annual Meeting of JBS

[3P-078] Endoplasmic reticulum stress sensor ATF6a forms two types of dimers via disulfide-bonding

^○Hibiki Koba, Shengyu Jin, Tetsuya Okada, Tokiro Ishikawa, Satoshi Ninagawa, kazutoshi Mori (Department of Biophysics, Graduate School of Science, Kyoto University)

The 92nd Annual Meeting of JBS

[3P-079] Effects of calnexin/calreticulin knockout and proline residue mutation of human prothrombin on the quality control of secretion proteins

^○Yoshihito Kimura, Mika Ozawa, Hiroaki Kawashima, Shigenobu Tone, Masato Tanaka (Tokyo Denki University)

The 92nd Annual Meeting of JBS